产品信息

蛋白质全称	神经束缚蛋白-2
别名	PPP1R9B, PPP1R6, PPP1R9, SPINO, Spn, protein phosphatase 1 regulatory subunit 9B, p130, Spinophilin, PP1bp134, Neurabin II, Neural tissue specific F actin binding protein II, Neurabin-II, Ppp1r9b
Uniprot ID	Q6R891
免疫原	小鼠Neurabin 2重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	90 kDa / 130 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠	1: 500-1: 1000	小脑

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背景

Neurabin-2 is a protein that in humans is encoded by the PPP1R9B gene. PPP1R9B has been shown to interact with PPP1CB, PPP1CA, Dopamine receptor D2, P16, PPP1CC, T-cell lymphoma invasion and metastasis-inducing protein 1 and PPP1R2. Neurabin-2, also known as Spinophilin, is a regulatory subunit of protein phosphatase-1 catalytic subunit and is highly enriched in dendritic spines, specialized protrusions from dendritic shafts that receive most of the excitatory input in the central nervous system.

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图像

WB检测Spinophilin/Neurabin 2蛋白(货号 YB111967). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.