产品信息

蛋白质全称	Spastin蛋白
别名	ADPSP, FSP2, KIAA1083, SPAST, Spastic paraplegia 4 protein, spastin, SPG4
Uniprot ID	Q9QYY8
免疫原	小鼠Spastin重组蛋白
抗体亚型	IgG
纯化方式	亲和纯化
亚细胞定位	膜, 内质网, 细胞质, 细胞骨架

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应用

应用	物种	稀释	阳性样品
IHC/IF 免疫组织化学/免疫荧光	小鼠, 大鼠	1: 500-1: 1500	结肠, 脑

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背景

ATP-dependent microtubule severing protein that specifically recognizes and cuts microtubules that are polyglutamylated.Preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold.Severing activity is not dependent on tubulin acetylation or detyrosination.Microtubule severing promotes reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation.It is critical for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia.

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图像

	IHC检测Spastin蛋白(货号 YB115434). 样品: 小鼠结肠, 4%多聚甲醛 (货号Y1112) 固定12-24小时. 抗原修复: Tris-EDTA抗原修复液(pH 9.0) (Y1214), 98℃, 20分钟. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 200稀释, 室温孵育1小时.
	IHC检测Spastin蛋白(货号 YB115434). 样品: 大鼠脑, 4%多聚甲醛 (货号Y1112) 固定12-24小时. 抗原修复: Tris-EDTA抗原修复液(pH 9.0) (Y1214), 98℃, 20分钟. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 200稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.