产品信息

蛋白质全称	MAP/微管亲和调节激酶3
别名	cTAK1, Cdc25C-associated protein kinase 1, ELKL motif kinase 2, EMK-2, Protein kinase STK10, Ser/Thr protein kinase PAR-1, Par-1a, Serine/threonine-protein kinase p78, Mark3, EMK2/MARK3 serine-threonine protein kinase
Uniprot ID	Q8VHF0
免疫原	人Mark3重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	84 kDa / 84 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	大鼠	1: 500-1: 1000	肺, 肾, 胃

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背景

Serine/threonine-protein kinase . Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU . Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus . Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1.

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图像

WB检测Mark3蛋白(货号 YB11987). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.