产品信息

蛋白质全称	肌球蛋白-7
别名	Myosin heavy chain 7, Myosin heavy chain slow isoform, MyHC-slow, MyHC-beta, CMD1S, CMH1, MPD1, MYH7, MyHC slow, MYHCB, Myosin 7, SPMD, SPMM, Beta myosin heavy chain
Uniprot ID	P12883
免疫原	KLH偶联的MYH7合成肽
抗体亚型	IgG
预测分子量./观测分子量.	223 kDa / 223 kDa
纯化方式	亲和纯化
亚细胞定位	细胞质

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 300-1: 600	心肌
IHC/IF 免疫组织化学/免疫荧光	人, 小鼠, 大鼠	1: 500-1: 1000	腿部 肌肉, 骨骼肌

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背景

This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading.

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图像

	WB检测Slow Skeletal Myosin Heavy chain蛋白(货号 YB112142). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 300稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.
	IHC检测Slow Skeletal Myosin Heavy chain蛋白(货号 YB112142). 样品: 大鼠骨骼肌, 4%多聚甲醛 (货号Y1112) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (Y1212), 98℃, 20分钟. —抗: 1: 600稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 200稀释, 室温孵育1小时.
	IHC检测Slow Skeletal Myosin Heavy chain蛋白(货号 YB112142). 样品: 大鼠骨骼肌, 4%多聚甲醛 (货号Y1112) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (Y1212), 98℃, 20分钟. —抗: 1: 600稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 200稀释, 室温孵育1小时.
	IHC检测Slow Skeletal Myosin Heavy chain蛋白(货号 YB112142). 样品: 小鼠骨骼肌, 4%多聚甲醛 (货号Y1112) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (Y1212), 98℃, 20分钟. —抗: 1: 600稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 200稀释, 室温孵育1小时.
	IF analysis of MYH7(YB112142) red. sample: Rat skeletal muscle, 4% PFA (Y1112) 12-24h. Antigen retrieval: TTris-EDTA buffer (pH 9.0) (Y1214), 98℃, 20min. Blocking buffer: 3% BSA in PBS (YC305021), RT, 30min. Primary antibody: MYH7(YB112142) red 1: 5000, 4℃ overnight.iF555-TSA(Y1244). MYH1 (YB112141) green 1: 1000, 4℃ overnight,488 conjugated Goat Anti-Rabbit IgG (H+L) (YB25314), 1: 400, RT, 1h.
	IF analysis of MYH7(YB112142) red. sample: Rat skeletal muscle, 4% PFA (Y1112) 12-24h. Antigen retrieval: TTris-EDTA buffer (pH 9.0) (Y1214), 98℃, 20min. Blocking buffer: 3% BSA in PBS (YC305021), RT, 30min. Primary antibody: MYH7(YB112142) red 1: 5000, 4℃ overnight.iF555-TSA(Y1244). MYH1 (YB112141) green 1: 1000, 4℃ overnight,488 conjugated Goat Anti-Rabbit IgG (H+L) (YB25314), 1: 400, RT, 1h.
	IF analysis of MYH7(YB112142) red. sample: Mouse skeletal muscle, 4% PFA (Y1112) 12-24h. Antigen retrieval: TTris-EDTA buffer (pH 9.0) (Y1214), 98℃, 20min. Blocking buffer: 3% BSA in PBS (YC305021), RT, 30min. Primary antibody: MYH7(YB112142) red 1: 5000, 4℃ overnight.iF555-TSA(Y1244). MYH1 (YB112141) green 1: 1000, 4℃ overnight,488-TSA(Y1242)

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.