产品信息

蛋白质全称	纤连蛋白
别名	FN, Fn1, CIG, Cold-insoluble globulin, FINC, GFND2, LETS, MSF, Migration stimulating factor, ED-B, FNZ, GFND, LETS, MSF, fibronectin 1, Cold insoluble globulin, ED B, Fibronectin, FN1, FNZ
Uniprot ID	P11276
免疫原	KLH偶联的小鼠Fibronectin合成肽
抗体亚型	IgG
预测分子量./观测分子量.	272 kDa / 270-300 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 250-1: 500	血浆

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背景

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.

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图像

WB检测Fibronectin蛋白(货号 YB112104). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 250稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.