产品信息

蛋白质全称	卷曲蛋白5
别名	FZD5, C2orf31, HFZ5, frizzled class receptor 5
Uniprot ID	Q9EQD0
免疫原	小鼠Frizzled 5重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	64 kDa / 50 kDa
纯化方式	亲和纯化
亚细胞定位	细胞膜

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 500-1: 1000	脑, 皮质, 小肠
IHC/IF 免疫组织化学/免疫荧光	人	1: 800-1: 1600	卵巢癌

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背景

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase.

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图像

WB检测Frizzled 5蛋白(货号 YB11633). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.