产品信息

蛋白质全称	SRSF蛋白激酶1
别名	SFRS protein kinase 1, SFRSK1, SR protein specific kinase 1, SRPK1, Serine/arginine-rich protein-specific kinase 1
Uniprot ID	O70551
免疫原	KLH偶联的小鼠SRPK1合成肽
抗体亚型	IgG
纯化方式	亲和纯化
亚细胞定位	细胞质, 内质网, 细胞核

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应用

应用	物种	稀释	阳性样品
IHC/IF 免疫组织化学/免疫荧光	小鼠	1: 400-1: 800	睾丸

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背景

SFRS protein kinase 1, SFRSK1, SR protein specific kinase 1, SRPK1SRPK1 (serine-arginine protein kinase 1) is a protein kinase that specifically phosphorylates proteins containing serine-arginine-rich domains. Its substrates include a family of SR proteins that are key regulators of mRNA AS (alternative splicing). SRPK1 may be localized both in the cytoplasm and nucleus and translocates between these compartments under various conditions (e.

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图像

IHC检测SRPK1蛋白(货号 YB113481). 样品: 小鼠睾丸, 4%多聚甲醛 (货号Y1112) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (Y1212), 98℃, 20分钟. —抗: 1: 600稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 200稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.