产品信息

蛋白质全称	双链RNA特异性腺苷脱氨酶
别名	DRADA, RNA adenosine deaminase 1, Adar, Adar1, 136kDa double stranded RNA binding protein, AGS6, Adenosine deaminase acting on RNA 1 A, Interferon inducible protein 4, RNA adenosine deaminase 1
Uniprot ID	Q99MU3
免疫原	小鼠ADAR1重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	130 kDa / 110 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 500-1: 1000	HEK293T细胞, Jurkat细胞, HepG2细胞

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背景

ADAR1 is also named as ADAR1, DSRAD, G1P1, IFI4. It convert selected adenosine residues into inosine in substrate RNAs containing a relatively short dsRNA region. The human ADAR1 gene specifies two size forms of RNA-specific adenosine deaminase, an interferon (IFN) inducible ∼150 kDa protein and a constitutively expressed N-terminally truncated ∼110 kDa protein, encoded by transcripts with alternative exon 1 structures that initiate from different promoters.

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图像

WB检测ADAR1蛋白(货号 YB111469). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.