产品信息

蛋白质全称	RNA聚合酶I亚基RPA2
别名	RNA polymerase I subunit 2, DNA-directed RNA polymerase I 135 kDa polypeptide, RPA135, Polr1b, Rpa2, Rpo1-2
Uniprot ID	P70700
免疫原	小鼠POLR1B重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	128 kDa / 128 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠	1: 1000-1: 2000	K562细胞, HepG2细胞, A431细胞

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背景

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft.

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图像

WB检测POLR1B蛋白(货号 YB112657). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.