产品信息

蛋白质全称	丝裂原活化蛋白激酶激酶激酶14
别名	MAP3K14, FTDCR1B, HS, HSNIK, NIK, Mitogen-activated protein kinase, kinase, kinase 14, Map3k14
Uniprot ID	Q9WUL6
免疫原	小鼠MAP3K14重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	104 kDa / 104 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 500-1: 1000	A549细胞, HeLa细胞, HepG2细胞

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背景

Mitogen-activated protein kinase kinase kinase 14 also known as NF-kappa-B-inducing kinase (NIK) is an enzyme encoded by the MAP3K14 gene. This protein is a serine/threonine protein-kinase. This kinase binds to TRAF2 and stimulates NF-κB activity. It is a critical kinase of the alternative NF-κB activation pathway. It shares sequence similarity with several other MAPKK kinases. It participates in an NF-κB-inducing signalling cascade common to receptors of the tumour-necrosis/nerve-growth factor (TNF/NGF) family and to the interleukin-1 type-I receptor.

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图像

WB检测NFkB Inducing Kinase NIK蛋白(货号 YB11779). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.