产品信息

蛋白质全称	G蛋白偶联受体161
别名	Gpr161, Gm208, RE2, G protein coupled receptor RE2
Uniprot ID	B2RPY5
免疫原	KLH偶联的小鼠GPR161合成肽
抗体亚型	IgG
预测分子量./观测分子量.	60 kDa / 65 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 500-1: 1000	心, 脑

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背景

GPR161 (also known as RE2) is an orphan G protein-coupled receptor and plays important roles in the Hh pathway. In the absence of Hh signals, GPR161 localizes to primary cilia and keeps the downstream GLI transcription factors in their repressor forms . Ciliary localization of GPR161 requires TULP3 and the IFT-A complex. In presence of SHH, GPR161 is removed from primary cilia and is internalized into recycling endosomes, preventing its activity and allowing activation of the Shh signaling.

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图像

WB检测GPR161蛋白(货号 YB111432). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.