
Anti-GNAT1 Rabbit pAb
- 50 μL
产品信息
蛋白质全称 | 鸟苷酸结合蛋白Gtα亚基1 |
别名 | Transducin alpha-1 chain, Gnat1, Gnat-1, CSNBAD3, Rod specific transducin, Transducin alpha-1 chain, GNATR, guanine nucleotide binding protein G(T) alpha 1 subunit |
Uniprot ID | P20612 |
免疫原 | 小鼠GNAT1重组蛋白 |
抗体亚型 | IgG |
预测分子量./观测分子量. | 40 kDa / 40 kDa |
纯化方式 | 亲和纯化 |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| WB 免疫印迹 | 小鼠, 大鼠 | 1: 3000-1: 5000 | 视网膜, 眼睛, 大脑 |
背景
Transducin is a 3-subunit guanine nucleotide-binding protein, which also an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.The transducin alpha subunits in rods and cones are encoded by separate genes.This gene is also expressed in other cells, and has been implicated in bitter taste transduction in rat taste cells. Mutations in this gene result in autosomal dominant congenital stationary night blindness.
图像
| WB检测GNAT1蛋白(货号 YB111596). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 3000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时. |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. |
注意:
1.本产品仅供研究使用.
2.建议用一抗稀释液(Y2036)稀释本产品.
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