产品信息

蛋白质全称	G蛋白偶联受体19
别名	Gpr19, GPR NGA, G protein coupled receptor GPR19
Uniprot ID	Q61121
免疫原	KLH偶联的小鼠GPCR GPR19合成肽
抗体亚型	IgG
预测分子量./观测分子量.	48 kDa / 55 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 500-1: 1000	脑, 大脑皮层

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背景

The G-protein-coupled receptor (GPCR) superfamily is comprised of an estimated 600-1, 000 members and is the largest known class of molecular targets with proven therapeutic value. GPR19 is an orphan receptor found primarily in the central nervous system. GPR19 was also found in ovary and testis and in human embryonic stem cells. GPR19 has been postulated to play a role in cancer development and increased levels of GPR19 mRNA were found in metastatic melanoma, small cell lung carcinoma and pancreas islet cell carcinoma.

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图像

WB检测GPCR GPR19蛋白(货号 YB111799). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.