产品信息

蛋白质全称	雷帕霉素靶蛋白复合体亚基LST8
别名	TORC subunit LST8, G protein beta subunit-like, Protein GbetaL, Mammalian lethal with SEC13 protein 8, mLST8, Gbl, Lst8, MGC111011
Uniprot ID	Q9DCJ1
免疫原	小鼠G protein beta subunit like/GBL重组蛋白
抗体亚型	IgG
纯化方式	亲和纯化
亚细胞定位	细胞质

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应用

应用	物种	稀释	阳性样品
IHC/IF 免疫组织化学/免疫荧光	人, 小鼠	1: 600-1: 1300	肺癌

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背景

G-protein beta subunit like (GBL protein) is a novel member of WD-40 protein family. It is a highly conserved cytosolic protein consisting of seven WD-40 repeating motifs. LST8 is a functional component of mTOR signaling complex (TORC1) and positively up-regulates the mTOR pathway. It interacts with the kinase domain of mTOR and stabilizes its interaction with raptor. LST8 participates in regulating cell growth and also in nutrient and growth factor-mediated mTOR signaling to S6K1.

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图像

IHC检测G protein beta subunit like/GBL蛋白(货号 YB111698). 样品: 人肺癌, 4%多聚甲醛 (货号Y1112) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (Y1212), 98℃, 20分钟. —抗: 1: 650稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 200稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.