产品信息

蛋白质全称	DNA复制许可因子MCM6
别名	Mis5 homolog, Mcm6, Mcmd6, Mis5, P105MCM
Uniprot ID	P97311
免疫原	KLH偶联的小鼠MCM6合成肽
抗体亚型	IgG
预测分子量./观测分子量.	93 kDa / 105 kDa
纯化方式	亲和纯化
亚细胞定位	染色体, 细胞核

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠	1: 1000-1: 2000	脾
IHC/IF 免疫组织化学/免疫荧光	人, 小鼠, 大鼠	1: 1800-1: 3600	扁桃体, 脾

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背景

The MCM genes were firstly identified in yeast defective in minichromosome maintenance and have since been shown to have roles in the progression of the cell cycle, and most of them are cell division control genes. MCM2-7 complex are suggested to be 'DNA licensing factors' which bind to the DNA after mitosis and enable DNA replication before being removed during S phase. Mini-chromosome maintenance 6 (MCM6) is one component of the MCM2-7 complex which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells.

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图像

	WB检测MCM6蛋白(货号 YB113004). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 2000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.
	WB检测MCM6蛋白(货号 YB113004). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 2000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.